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Literature summary extracted from

  • Bresler, M.M.; Rosser, S.J.; Basran, A.; Bruce, N.C.
    Gene cloning and nucleotide sequencing and properties of a cocaine esterase from Rhodococcus sp. strain MB1 (2000), Appl. Environ. Microbiol., 66, 904-908.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.84 the cocE coding sequence was subcloned into the pCFX1 expression plasmid and expressed in Escherichia coli. sequence comparison suggests that cocE encodes a serine esterase Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.84 1.33
-
 cocaine pH 7.5, 30°C Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.84 62128
-
 1 * 62128, calculated from sequence Rhodococcus sp.
3.1.1.84 65000
-
 gel filtration Rhodococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.84 cocaine + H2O Rhodococcus sp. Rhodococcus sp. MB1 is capable of utilizing cocaine as a sole source of carbon and nitrogen for growth. The organism lives in the rhizosphere soil of the tropane alkaloid-producing plant Erythroxylum coca. The cocaine esterase initiates degradation of cocaine, which is hydrolyzed to ecgonine methyl ester and benzoate. Both of these esterolytic products are further metabolized by Rhodococcus sp. strain MB1. This activity is inducible in Rhodococcus sp. strain MB1, since no cocaine esterase activity is observed in cells grown on 15 mM succinate as a sole source of carbon ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O Rhodococcus sp. MB1 Rhodococcus sp. MB1 is capable of utilizing cocaine as a sole source of carbon and nitrogen for growth. The organism lives in the rhizosphere soil of the tropane alkaloid-producing plant Erythroxylum coca. The cocaine esterase initiates degradation of cocaine, which is hydrolyzed to ecgonine methyl ester and benzoate. Both of these esterolytic products are further metabolized by Rhodococcus sp. strain MB1. This activity is inducible in Rhodococcus sp. strain MB1, since no cocaine esterase activity is observed in cells grown on 15 mM succinate as a sole source of carbon ecgonine methyl ester + benzoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.84 Rhodococcus sp. Q9L9D7
-
-
3.1.1.84 Rhodococcus sp. MB1 Q9L9D7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.84 recombinant enzyme Rhodococcus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.84 culture condition:cocaine-grown cell Rhodococcus sp. MB1 is capable of utilizing cocaine as a sole source of carbon and nitrogen for growth Rhodococcus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.84 43.92
-
-
 Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.84 cocaine + H2O
-
 Rhodococcus sp. ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O Rhodococcus sp. MB1 is capable of utilizing cocaine as a sole source of carbon and nitrogen for growth. The organism lives in the rhizosphere soil of the tropane alkaloid-producing plant Erythroxylum coca. The cocaine esterase initiates degradation of cocaine, which is hydrolyzed to ecgonine methyl ester and benzoate. Both of these esterolytic products are further metabolized by Rhodococcus sp. strain MB1. This activity is inducible in Rhodococcus sp. strain MB1, since no cocaine esterase activity is observed in cells grown on 15 mM succinate as a sole source of carbon Rhodococcus sp. ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O
-
 Rhodococcus sp. MB1 ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O Rhodococcus sp. MB1 is capable of utilizing cocaine as a sole source of carbon and nitrogen for growth. The organism lives in the rhizosphere soil of the tropane alkaloid-producing plant Erythroxylum coca. The cocaine esterase initiates degradation of cocaine, which is hydrolyzed to ecgonine methyl ester and benzoate. Both of these esterolytic products are further metabolized by Rhodococcus sp. strain MB1. This activity is inducible in Rhodococcus sp. strain MB1, since no cocaine esterase activity is observed in cells grown on 15 mM succinate as a sole source of carbon Rhodococcus sp. MB1 ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 additional information the cocaine esterase displays low levels of activity with 20 mM atropine, a structurally related tropane alkaloid Rhodococcus sp. ?
-
 ?
3.1.1.84 additional information the cocaine esterase displays low levels of activity with 20 mM atropine, a structurally related tropane alkaloid Rhodococcus sp. MB1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.84 monomer 1 * 62128, calculated from sequence Rhodococcus sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.84 cocE
-
 Rhodococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.84 30
-
 assay at Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.84 7.5
-
 assay at Rhodococcus sp.